polymorphism of insulin-like growth factor binding protein-3 (igfbp-3) gene associated with growth traits in cyprinus carpio
نویسندگان
چکیده
the genetic polymorphism of insulin-like growth factor binding protein ( igfbp-3 ) and its association with the growth traits namely condition factor, body weight, and total length were investigated in common carp cyprinus carpio using pcr- single strand conformation polymorphism (sscp) technique. caudal fin clips from 120 c. carpio , aging 5 months, were used in this study and a 330 bp dna fragment was amplified from 5'-utr of carp igfbp-3 . sscp genotyping yielded seven different banding patterns namely a, b, c, d, e, f, and g with frequencies of 14.16, 12.5, 21.67, 9.17, 11.67, 10.0, and 20.84, respectively. the obtained results showed a relatively high polymorphic behavior of 5'-utr regulatory region of studied locus; therefore it shows the importance of this region in regulation of transcription of igfbp-3 gene in carp. marker-trait association showed significant relationships between the detected genotypes and condition factor (cf). igfbp-3 gene polymorphism in joint with cf signifies relatively high genetic diversity and significant potential for marker-assisted selection in this species.
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عنوان ژورنال:
iranian journal of ichthyologyجلد ۲، شماره ۳، صفحات ۱۷۲-۱۷۶
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